| 產品編號 | IHC0636 |
| 英文名稱 | PMCA1 Ready-To-Use IHC Kit |
| 中文名稱 | 細胞膜鈣ATP酶1即用型免疫組化試劑盒 |
| 別 名 | 2810442I22Rik; ATP2B1; E130111D10Rik; PMCA1; Pmca1a; Pmca1b; Pmca1c; PMCA1kb |
| 適用樣品基質 | FFPE tissue |
| 保存條件 | Please store components at the temperatures indicated on the individual tube labels. The kit is stable for 6 months from the date of receipt. |
| 產品介紹 |
The calcium pump of the plasma membrane, termed PMCA ATPase, pumps calcium from the cytosol to the extracellular space. This membrane-bound enzyme is related to a number of other ATPases including the SERCA ATPase and the sodium/potassium pump. There are four different genes encoding PMCA ATPase and studies have revealed 20 isoforms of the pump generated by alternate splicing of the primary gene products. mRNA distribution studies show that gene products 1 and 4 are transcribed in most tissues, however, products 2 and 3 are more tissue specific. Transcription of the splicing variants has also been found to be tissue specific. In the pancreas, where insulin secretion is calcium dependent, the beta cells only express the 4b isoform, however the alpha and gamma cells express both 4a and 4b isoforms. Studies have also shown that different splice variants have different affinities for calcium and calmodulin. SWISS: P20020 Gene ID: 490 |
| 產品圖片 |
Immunohistochemical analysis of paraffin embedded Mouse stomach tissue slide using IHC0636 (PMCA1 Kit).
Immunohistochemical analysis of paraffin embedded Human stomach tissue slide using IHC0636 (PMCA1 Kit).
Immunohistochemical analysis of paraffin embedded Rat stomach tissue slide using IHC0636 (PMCA1 Kit).
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
