| 產品編號 | bs-8541R |
| 英文名稱 | CLEC 4E Rabbit pAb |
| 中文名稱 | C型凝集素4家族E抗體 |
| 別 名 | C type lectin domain family 4 member E; C type lectin superfamily member 9; C-type(calcium dependent carbohydrate recognition domain) lectin superfamily member 9; C-type lectin domain family 4 member E; C-type lectin superfamily member 9; CLC4E_HUMAN; CLEC 4E; CLEC4E Clec4e; CLECSF9; Macrophage inducible C type lectin; Macrophage-inducible C-type lectin; MINCLE. |
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Specific References (6) | bs-8541R has been referenced in 6 publications.
[IF=11.864] Darja Andreev. et al. Osteocyte necrosis triggers osteoclast-mediated bone loss through macrophage-inducible C-type lectin. J Clin Invest. 2020 Sep;130(9):4811-4830 IF ; Mouse.
[IF=5.72] He, Yue, et al. "Mincle/Syk Signaling Pathway Contributes to Neuroinflammation after Subarachnoid Hemorrhage in Rats." Stroke; a journal of cerebral circulation 46.8 (2015): 2277. WB, IHC ; Rat.
[IF=4.302] Wang Y et al. Microglial Mincle receptor in the PVN contributes to sympathetic hyperactivity in acute myocardial infarction rat.(2018) J Cell Mol Med. Oct 24. IF,WB ; Rat.
[IF=3.201] Yuki Sato. et al. Limited Role of Mincle in the Host Defense against Infection with Cryptococcus deneoformans. Infect Immun. 2020 Oct;88(11):e00400-20 IHC ; Mouse.
[IF=2.234] Liu?XY et al. Acupuncture through (DU20) to (GB7) mitigates neurological impairment after intracerebral hemorrhage. Neural Regen Res. 2018 Aug;13(8):1425-1432. IHC-P&WB ; Rat.
[IF=1.166] Yu GR et al. Mincle in the innate immune response of mice fungal keratitis.Int J Ophthalmol. 2018 Apr 18;11(4):539-547. IF ; Mouse.
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| 研究領域 | 細胞生物 信號轉導 細胞凋亡 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human (predicted: Mouse,Rat,Cow,Horse) |
| 產品應用 | Flow-Cyt=2μg/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 25 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CLECSF9: 51-150/219 <Extracellular> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
C-type lectin that functions as cell-surface receptor for a wide variety of ligands such as damaged cells, fungi and mycobacteria. Plays a role in the recognition of pathogenic fungi, such as Candida albicans. The detection of mycobacteria is via trehalose 6,6'-dimycolate (TDM), a cell wall glycolipid. Specifically recognizes alpha-mannose residues on pathogenic fungi of the genus Malassezia. Recognizes also SAP130, a nuclear protein, that is released by dead or dying cells. Transduces signals through an ITAM-containing adapter protein, Fc receptor gamma chain /FCER1G. Induces secretion of inflammatory cytokines through a pathway that depends on SYK, CARD9 and NF-kappa-B. Function: C-type lectin that functions as cell-surface receptor for a wide variety of ligands such as damaged cells, fungi and mycobacteria. Plays a role in the recognition of pathogenic fungi, such as Candida albicans. The detection of mycobacteria is via trehalose 6,6'-dimycolate (TDM), a cell wall glycolipid. Specifically recognizes alpha-mannose residues on pathogenic fungi of the genus Malassezia. Recognizes also SAP130, a nuclear protein, that is released by dead or dying cells. Transduces signals through an ITAM-containing adapter protein, Fc receptor gamma chain /FCER1G. Induces secretion of inflammatory cytokines through a pathway that depends on SYK, CARD9 and NF-kappa-B. Subunit: Monomer. Homodimer. Subcellular Location: Membrane. Similarity: Contains 1 C-type lectin domain. SWISS: Q9ULY5 Gene ID: 26253 Database links: Entrez Gene: 26253 Human Omim: 609962 Human SwissProt: Q9ULY5 Human Unigene: 236516 Human |
| 產品圖片 |
Blank control:THP-1.
Primary Antibody (green line): Rabbit Anti-CLEC 4E antibody (bs-8541R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Black line : Positive blank control (Hela); Negative blank control (A431）
Green line : Primary Antibody (Rabbit Anti-CLEC 4E antibody (bs-8541R) )
Orange line：Isotype Control Antibody (Rabbit IgG) .
Blue line : Secondary Antibody (Goat anti-rabbit IgG-PE)/(Goat anti-rabbit IgG-AF488)
Hela（Positive）and A431（Negative control）cells (black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CLEC 4E Antibody(bs-8541R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Blank control: Hela(blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice.
Isotype Control Antibody: Rabbit IgG(orange) ;
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue),
Dilution: 1:200 in 1 X PBS containing 0.5% BSA ;
Primary Antibody Dilution: 5μg in 100 μL1X PBS containing 0.5% BSA(green).
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
